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DISPAA Dipartimento di Scienze delle Produzioni Agroalimentari e dell'Ambiente
Home page > Department > Staff > Personal Pages > Laura Giagnoni

Laura Giagnoni

Personal data


  italy

Laura Giagnoni

Postdoctoral Research fellow

Department of Agrifood Production and Environmental Sciences (DISPAA)

Piazzale delle Cascine, 18 50144 – Firenze (FI)


e-mail:
laura.giagnoniatunifi.it

 

 

Academic Education



PhD in Soil Science and Climatology – University of Florence, Florence, Italy
Date: Apr 08 2011
Field of study: Soil Science, soil chemistry and soil biochemistry 
PhD thesis: Proteomic analysis of Cupriavidus metallidurans: a model for soil proteomic studies”
Tutor: Prof. Loretta Landi

Master degree in Biology – University of Florence, Florence, Italy
Date: July 18 2007
Field of study: Molecular Biology, microbiology, environmental microbiology
Master thesis: Antarctic bacteria plasmids sequencing and bioinformatic analysis”
Tutor: Prof. Renato Fani

 

 

Research interests


Field of Study

  • Soil pollution and remediation techniques: phytoremediation, bioremediation, co-composting
  • Soil organic matter turnover and stabilization
  • Soil microbial ecology
  • Soil and microbial proteomics
  • Soil biochemistry
  • Soil chemistry
  • Soil microbial molecular biology: soil microbial communities analysis by GeoChip (microarray)
  • DNA and RNA analysis: metagenomics, metatrascriptomics
  • Soil volatile organic compounds (VOCs) analysis by PTR-TOF
  • Ag-nanoparticles

 

 

Professional background


From 01.12.2017 to present             
Postdoctoral research position at University of Florence, Dept. Agrifood Production and Environmental Science, Florence, Italy
Main research activities: Soil chemical and biochemical analysis, environmental pollution analysis, various phytoremediation, bioremediation and co-composting techniques, rhizosphere analysis, soil VOCs analysis, soil microbial communities analysis by GeoChip 

From 01.07.2014 to 14.10.2016                   
Postdoctoral research position at University of Florence, Dept. Agrifood Production and Environmental Science, Florence, Italy
Main research activities: Soil chemical and biochemical analysis, environmental pollution analysis, various phytoremediation, bioremediation and co-composting techniques, rhizosphere analysis, soil VOCs analysis, soil microbial communities analysis by GeoChip, soil proteomics

From 01.07.2013 to 31.06.2014                   
Postdoctoral research position at University of Florence, Dept. Agrifood Production and Environmental Science, Florence, Italy
Main research activities: Soil Proteomics, soil chemical and biochemical analysis, environmental pollution analysis, various phytoremediation, bioremediation and co-composting techniques, rhizosphere analysis, interaction soil/plant in rhizosphere, soil VOCs analysis 

From 01.01.2012 to 31.06.2013       
Postdoctoral research position at University of Florence, Dept. Plant, Soil and Environmental Sc., Florence, Italy
Main research activities: Soil Proteomics, soil chemical and biochemical analysis, environmental pollution analysis, various phytoremediation, bioremediation and co-composting techniques, rhizosphere analysis, interaction soil/plant in rhizosphere, plant growth in rhizobox 

From 01.01.2011 to 31.12.2011       
Postdoctoral research position at University of Florence, Dept. Plant, Soil and Environmental Sc., Florence, Italy
Main research activities: Soil Proteomics, soil chemical and biochemical analysis, environmental pollution analysis, various phytoremediation techniques use

 

 

Research experience in European laboratories


From 01.07.2013 to 31.07.2013
Research grant (SISS fellowship grant) at Institute of Agrophysics, Lublin, Poland
Main research activities: Microbial and chemical analysis in artificial soil, Ag-nanoparticles preparation and analysis in artificial soil

From 01.08.2012 to 31.08.2012                   
Research training - University of Wien- Institute of Soil Research, Wien, Austria
Main research activities: Rhizobox use and plant growth in green house

 

 

Laboratory experience and skills


Molecular Biology techniques:
Bacterial culture growth, Microbial availability by Colony forming Unit (CFU), Microbial DNA extraction (genome and plasmids), Soil RNA/DNA extraction, PCR, Retrotrascription, DNA Cloning, ligand and transformation, Terminal Restriction Fragment Length Polymorphism (TRLFP), Agarose gel electrophoresis, DGGE, Genomic database search, Soil microbial biomass

Biochemistry techniques:
Soil and microbial protein extraction,SDS-PAGE and 2-DE electrophoresis, Gel image analysis by Image Master 2D Platinum,Mass Spectrometry sample preparation (MALDI-TOF and ORBITRAP),Proteomics database search and protein identification (software Bioworks and Mascot)

Soil Chemical, Biochemical techniques and agronomic plants management:
Soil chemical analysis: pH, conductivity, organic carbon, cation exchange capacity, ammonium and nitrate ions by ion electrode, total phosphorus, Olsen/Bray&Kurtz methods, soil organic matter determination, soil enzyme activities assay, soil toxicity by BioTox, rhizobox management for plant growth in climatic chamber, ammonium and nitrate ion content in soil solution, use of N15stable isotope as N source for agronomic plant (Maize), volatile soil organic compounds (VOCs) analysis by PTR-TOF

Ag Nanoparticles:
Ag-nanoparticles preparation, Zeta potential and size determination by Zetasizer, Ag-nanoparticles spectrum analysis by spectrophotometer

 

 

Conferences attendance


From 2008 to present
Attendance at many national and international conferences in Soil and Environmental Sciences and Proteomics

 

 
last update: 28-May-2018
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